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methylation sensitive restriction enzyme apeki  (New England Biolabs)


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    Structured Review

    New England Biolabs methylation sensitive restriction enzyme apeki
    Methylation Sensitive Restriction Enzyme Apeki, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 316 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/methylation+sensitive+restriction+enzymes/ApeKI/pm42080968-293-7-11
    Average 96 stars, based on 316 article reviews
    methylation sensitive restriction enzyme apeki - by Bioz Stars, 2026-09
    96/100 stars

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    Modification:

    Article Title: High-resolution genetic maps and QTL mapping applications reveal polycystin gene involvement in oyster shell formation
    Article Snippet: Amplified fragments were separated by gel electrophoresis, with a 50-bp DNA ladder (Takara) used as reference markers. .. Libraries were prepared using modified GBS methods according to the method of Qi et al. A total of 200 ng genomic DNA from each individual was digested in a 30 μl reaction using methylation sensitive restriction enzymes 8U MspI and 8U PstI HF (NEB) at 37 °C for 2 h. The restriction enzymes were inactivated by incubation at 75 °C for 20 min. A total of 20 μl of the restriction digest was mixed with 1.5 μl of common MspI adapater, 1 μl of barcoded PstI HF adapter (stock: 0.1μM), 200U of T4 DNA ligase (NEB) and 4 μl of 10 × T4 DNA ligase buffer. .. Ligation was performed at 22 °C for 2 h, fragments < 300bp were eliminated using Sera-Mag Speed Beads (GE Healthcare Life Sciences) (0.7 volumes) at room temperature for 5 min. A magnetic stand was used to separated beads from the supernatant, and then washed three times with 200 μl of 70% ethanol.

    Methylation:

    Article Title: High-resolution genetic maps and QTL mapping applications reveal polycystin gene involvement in oyster shell formation
    Article Snippet: Amplified fragments were separated by gel electrophoresis, with a 50-bp DNA ladder (Takara) used as reference markers. .. Libraries were prepared using modified GBS methods according to the method of Qi et al. A total of 200 ng genomic DNA from each individual was digested in a 30 μl reaction using methylation sensitive restriction enzymes 8U MspI and 8U PstI HF (NEB) at 37 °C for 2 h. The restriction enzymes were inactivated by incubation at 75 °C for 20 min. A total of 20 μl of the restriction digest was mixed with 1.5 μl of common MspI adapater, 1 μl of barcoded PstI HF adapter (stock: 0.1μM), 200U of T4 DNA ligase (NEB) and 4 μl of 10 × T4 DNA ligase buffer. .. Ligation was performed at 22 °C for 2 h, fragments < 300bp were eliminated using Sera-Mag Speed Beads (GE Healthcare Life Sciences) (0.7 volumes) at room temperature for 5 min. A magnetic stand was used to separated beads from the supernatant, and then washed three times with 200 μl of 70% ethanol.

    Article Title: Characterization of NANOS Expression and Function in Human Germ Cells
    Article Snippet: .. each genomic DNA sample was divided equally: 1) control (incubated with buffers), 2) methylation sensitive restriction enzymes (Not1, HhaI, and HpaII; New England Biolabs) and 3) methylation-dependent restriction enzymes (McrBC; New England Biolabs). .. 1/30 of the digested genomic DNA was used as the input for a qPCR reaction using ABI Power SYBR green Master Mix (Applied Biosystems).

    Article Title: Lack of Phosphotyrosine Phosphatase SHP-1 Expression in Malignant T-Cell Lymphoma Cells Results from Methylation of the SHP-1 Promoter
    Article Snippet: Genomic DNA extraction was performed with 2 × 10 7 cells using Qiagen Genomic DNA kit (Qiagen). .. For PCR, DNA was digested with methylation sensitive restriction enzymes ( Hpa II, Eag I or Nae I; New England Biolabs, Beverly, MA) for 4 hours at 37°C. ..

    Incubation:

    Article Title: High-resolution genetic maps and QTL mapping applications reveal polycystin gene involvement in oyster shell formation
    Article Snippet: Amplified fragments were separated by gel electrophoresis, with a 50-bp DNA ladder (Takara) used as reference markers. .. Libraries were prepared using modified GBS methods according to the method of Qi et al. A total of 200 ng genomic DNA from each individual was digested in a 30 μl reaction using methylation sensitive restriction enzymes 8U MspI and 8U PstI HF (NEB) at 37 °C for 2 h. The restriction enzymes were inactivated by incubation at 75 °C for 20 min. A total of 20 μl of the restriction digest was mixed with 1.5 μl of common MspI adapater, 1 μl of barcoded PstI HF adapter (stock: 0.1μM), 200U of T4 DNA ligase (NEB) and 4 μl of 10 × T4 DNA ligase buffer. .. Ligation was performed at 22 °C for 2 h, fragments < 300bp were eliminated using Sera-Mag Speed Beads (GE Healthcare Life Sciences) (0.7 volumes) at room temperature for 5 min. A magnetic stand was used to separated beads from the supernatant, and then washed three times with 200 μl of 70% ethanol.

    Article Title: Characterization of NANOS Expression and Function in Human Germ Cells
    Article Snippet: .. each genomic DNA sample was divided equally: 1) control (incubated with buffers), 2) methylation sensitive restriction enzymes (Not1, HhaI, and HpaII; New England Biolabs) and 3) methylation-dependent restriction enzymes (McrBC; New England Biolabs). .. 1/30 of the digested genomic DNA was used as the input for a qPCR reaction using ABI Power SYBR green Master Mix (Applied Biosystems).

    Control:

    Article Title: Characterization of NANOS Expression and Function in Human Germ Cells
    Article Snippet: .. each genomic DNA sample was divided equally: 1) control (incubated with buffers), 2) methylation sensitive restriction enzymes (Not1, HhaI, and HpaII; New England Biolabs) and 3) methylation-dependent restriction enzymes (McrBC; New England Biolabs). .. 1/30 of the digested genomic DNA was used as the input for a qPCR reaction using ABI Power SYBR green Master Mix (Applied Biosystems).

    Polymerase Chain Reaction:

    Article Title: Lack of Phosphotyrosine Phosphatase SHP-1 Expression in Malignant T-Cell Lymphoma Cells Results from Methylation of the SHP-1 Promoter
    Article Snippet: Genomic DNA extraction was performed with 2 × 10 7 cells using Qiagen Genomic DNA kit (Qiagen). .. For PCR, DNA was digested with methylation sensitive restriction enzymes ( Hpa II, Eag I or Nae I; New England Biolabs, Beverly, MA) for 4 hours at 37°C. ..



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